Microbiology
Arezoo Jahanbin; Mostafa Entezari; Erfan Bardideh
Abstract
Aim: Droplets and aerosols are the main source of transmission and rapid spread of COVID-19 worldwide. Topical mouthwashes and oronasal irrigation are recommended as ways of preventing airborne transmission to health care professionals. This meta-analysis aimed to assess the changes of COVID 19 viral ...
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Aim: Droplets and aerosols are the main source of transmission and rapid spread of COVID-19 worldwide. Topical mouthwashes and oronasal irrigation are recommended as ways of preventing airborne transmission to health care professionals. This meta-analysis aimed to assess the changes of COVID 19 viral load after administration of different mouthwashes.Methods: An electronic search was undertaken including the following databases: Medline/PubMed, Web of Science, Scopus, and Embase. Search for grey literature, and hand search for relevant studies was also performed. The quality of included randomized clinical trials (RCTs) were assessed using the Cochrane Collaborations. Afterward, the relevant data from the included studies was extracted. Inverse-variance random-effects meta-analysis was performed to compare the effects of different types of mouthwashes on COVID viral presence.Results: After screening 1437 studies, 10 randomized clinical trials were finally selected. A total of 884 patients were assessed in these studies. The meta-analysis revealed the Cyclic Threshold (CT) values increased after the use of mouthwashes (MD=2.00, 95%CI= 0.51, 3.49, P<0.05). Considering the CT values are inversely correlated to the viral load, the result means the use of mouthwashes decreases the viral load in the saliva. This study also showed that increase of CT values was statistically significant for Povidone Iodine mouthwash (MD=4.08, 95%CI= 0.13, 8.02, P<0.05); however, Cetylpyridinium Chloride and Chlorhexidine Gluconate mouthwashes non-significantly (P value>0.05) reduced viral load in the saliva of patients with COVID 19.Conclusion: According to the result of this study, the use of mouthwashes reduces the viral load of saliva in patients with COVID 19. In addition to basic precautions for preventing the transmission of COVID 19, using mouthwashes may be a reasonable way to decrease the risk of disease transmission to medical staff.
Dental Materials
Hooman Shafaee; Fahimeh Farzanegan; Mohammad Sadegh Nazari; Erfan Bardideh; Jalil Tavakkol Afshari; Fatemeh Azani
Abstract
Background: The aim of study was the biological assessment of cultured RAW264 macrophage exposed to nano amorphous calcium phosphate particles by analyzing of cytotoxicity and genotoxicity tests.Methods: Nanoamorphus Calcium Phosphate particles were produced by sol-gel method, then particle size and ...
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Background: The aim of study was the biological assessment of cultured RAW264 macrophage exposed to nano amorphous calcium phosphate particles by analyzing of cytotoxicity and genotoxicity tests.Methods: Nanoamorphus Calcium Phosphate particles were produced by sol-gel method, then particle size and hemogenicity was analyzed by XRD (X ray Diffraction). Cytotoxicity of nanoparticles was determinated with mouse RAW264 macrophage. The cells cultured in 37°c in DMEM medium 96 parts plates with concentration of 10000 cells in each part for 72 hours, then the medium removed and the second medium added to cells containing different concentrations of Nano particle (0, 200 and 400µg/ml). After 24 hours of incubation, MTT assay and Annexin V were used for assessing cell viability and apoptosis.Results: The apoptosis insignificantly increased in macrophages with 200 and 400µg/ml NACP and for cytotoxicity, cell viability for control, 200µg and 400µg groups were 100,107,103 percent.Discussion: NACP has no cytotoxicity and genotoxicity, so it can be used as non-toxic and beneficial material for clinical use.
Hooman Shafaee; Bahareh Mazloumhoseini; Erfan Bardideh; Maliheh Dadgarmoghaddam
Abstract
Context: Maxillary deficiency can lead to the reduction of airway space and increase the chances of development of obstructive airway disorders. Facemask therapy is one of the main treatment protocols in developing maxillary deficient patients.Objectives: The purpose of this systematic review and meta-analysis ...
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Context: Maxillary deficiency can lead to the reduction of airway space and increase the chances of development of obstructive airway disorders. Facemask therapy is one of the main treatment protocols in developing maxillary deficient patients.Objectives: The purpose of this systematic review and meta-analysis was to assess the changes in the airway dimensions after face-mask therapy in both cleft lip and palate and non-cleft patients.Methods: A systematic search in different electronic databases (EMBASE, Pubmed, Cochrance Central register of controlled trials), IADR proceedings and a hand search by October 2020 were conducted and a meta-analysis and systematic review was performed.Results: In patients without cleft lip and palate, upper pharyngeal width was significantly increased by mean of 2.05 mm (CI = 95%, 0.61 - 3.50) following facemask therapy in comparison to patients who did not receive the treatment. Other upper pharyngeal (nasopharyngeal) measurements also showed a statistically significant improvement after therapy: S-PNS by 4.64 mm (CI = 95%, 3.34 - 5.94), AD1-PNS by 3.81 mm (CI = 95%, 2.40 - 5.21), AD2-PNS by 2.90 mm (CI = 95%, 0.13 - 5.67) and Pm’-SPL by 2.53 (CI = 95%, 0.54 - 4.51). Lower pharyngeal measurments did not show any significant changes after the treatment (P > 0.05). In the analysis of studies with 3D imaging modalities, upper pharyngeal volume was also significantly increased by 499.29 mm3 (CI = 95%, 69.58-929.00) after the treatment. In addition, a review of articles that included cleft lip and palate patients also showed after the treatment, the upper pharyngeal measurements all showed a significant improvement (P < 0.05), whereas the oropharyngeal region was relatively stable.Conclusions: In maxillary deficient patients with or without an orofacial cleft, facemask therapy can improve the nasopharyngeal area dimensions; however, this treatment protocol appears not to have an effect on the oropharyngeal area of the airway tract.
